Direct Fabrication and Harvesting of Monodisperse, Shape-Specific Nanobiomaterials

摘要: A versatile "top-down" method for the fabrication of particles, Particle Replication In Nonwetting Templates (PRINT), is described which affords absolute control over particle size, shape, and composition. This technique general enough to fabricate particles with a variety chemical structures, yet delicate be compatible sophisticated biological agents. Using PRINT, we have fabricated monodisperse poly(ethylene glycol diacrylate), triacrylate resin, poly(lactic acid), poly(pyrrole). Monodisperse populations, ranging from sub-200 nm nanoparticles complex micron-scale objects, been harvested. PRINT uses low-surface energy, chemically resistant fluoropolymers as molding materials, eliminates formation residual interconnecting film between molded objects. Until now, presence this has largely prevented using soft lithography. Importantly, demonstrated that simple, straightforward encapsulation important bioactive agents, including proteins, DNA, small-molecule therapeutics, indicates can used next-generation particulate drug-delivery