Role of Slowed Ca2+Transient Decline in Slowed Relaxation During Myocardial Ischemia
作者： Jessica M Halow , Vincent M Figueredo , David M Shames , S.Albert Camacho , Anthony J Baker
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摘要: Abstract The goal of this study was to test the hypothesis that during myocardial ischemia, slowing Ca 2+ transient decline causes slowed relaxation. Our approach monitor pressure and transients in isovolumic rat hearts control low flow ischemia conditions. In addition, we experimentally using cyclopiazonic acid (CPA) inhibit sarcoplasmic reticulum -ATPase (SERCA, most important pump for rapidly transporting out cytosol). Using 9 μ m CPA normoxia, were able reproduce relaxation found ischemia. time constants cytosolic [Ca ] ( τ P respectively) with (78±5 ms 64±3 ms) similar those (89±12 72±10 ms, mean±SEM, n =7) considerably greater than controls (41±3 25±2 =14, v These findings are consistent both Consistent conclusion, a simple mathematical model relate also suggests can be explained by decline. This impaired uptake is major injury causing
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