Single Molecule Force Spectroscopy to Compare Natural versus Artificial Antibody-Antigen Interaction.
作者： Congzhou Wang , Rong Hu , Jeremiah J. Morrissey , Evan D. Kharasch , Srikanth Singamaneni
关键词:
摘要: Biorecognition is central to various biological processes and finds numerous applications in virtually all areas of chemistry, biology, medicine. Artificial antibodies, produced by imprinting synthetic polymers, are designed mimic the recognition capability natural while exhibiting superior thermal, chemical, environmental stability compared their counterparts. The binding affinity artificial antibodies antigens characterizes biorecognition ability these nanoconstructs replace elements. However, a quantitative study an antibody antigen, especially at molecular level, still lacking. In this study, using atomic force microscopy-based spectroscopy, authors show that antigen (hemoglobin) weaker than antibody. fine difference interactions manifests into significant bioanalytical parameters biosensors based on
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