Detection of okadaic acid esters in the hexane extracts of Spanish mussels.

摘要: Two types of low polar derivatives OA and dinophysitoxins have been reported in shellfish or phytoplankton: 7-0-acyl esters containing a fatty acyl group attached through the 7-OH diol which carboxylic toxins has esterified. These compounds cannot be directly detected by liquid chromatography fluorimetric detection as 9-anthryldiazomethane derivatives, owing first case to their polarity high molecular weight, second because they All them must hydrolysed before derivatization Adam corresponding non-acylated toxins. In Lee procedure, after extraction digestive glands with 80% methanol, liquid-liquid partition non-polar solvent such hexane is carried out order remove lipids. The presence was investigated Spanish mussels confirmed layer, usually discarded conventional procedures, analysis alkaline hydrolysis products. A preferred solubilization these like reported. inclusion hydrolytic step extract general procedure suggested monitor contribution diarrhoetic poisons (DSPs) total DSP toxicity. This report DSPs other than DTX2 mussels.