Glycolipid-based nanostructures with thermal-phase transition behavior functioning as solubilizers and refolding accelerators for protein aggregates.
作者： N. Kameta , T. Matsuzawa , K. Yaoi , J. Fukuda , M. Masuda
DOI: 10.1039/C7SM00310B
关键词:
摘要: The self-assembly of synthetic glycolipids produced nanostructures such as vesicles and nanotubes consisting bilayer membranes, which underwent a gel-to-liquid crystalline thermal phase transition. Vesicles formed at temperatures above the transition (Tg-l) could solubilize aggregates denatured proteins by trapping them in fluid membranes. Cooling to below Tg-l caused morphological transformation into that accompanied from solid state. This phenomenon allowed trapped be quickly released bulk solution simultaneously facilitated refolding proteins. efficiency strongly depended on electrostatic attraction between membranes Because long shape (>400 nm) nanotubes, simple membrane filtration through pore size 200 nm led complete separation recovery refolded (3-9 sizes).
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