High-performance thin-layer chromatography with densitometry for the determination of ciprofloxacin and impurities in drugs.

摘要: A thin-layer chromatographic (TLC)-densitometric method has been developed for identification and quantification of ciprofloxacin (R f = 0.61) an ethylenediamine compound 0.42), a desfluoro 0.48), by-compound 0.53), fluoroquinolonic acid 0.68) as degradation products in pharmaceutical preparations. By using chloroform-methanol-25% ammonia (43 + 43 14, v/v/v) the mobile phase silica gel 60 F 2 5 4 high-performance TLC plates stationary phase, it was possible to separate individual constituents that, when subjected ultraviolet (UV) densitometric analysis at 330 nm 277 other compounds, gave well peaks allowing easy qualitative quantitative analyses. DMSO-methanol (1 1) used extract drug constituents. The showed high sensitivity (limit detection 10 44 ng), wide linearity range (3 20 μg/mL), good precision (2.32 6.46% relative standard deviation) accuracy (percentage recoveries 98.62 101.52%)