Isolation of Human Immunodeficiency Virus Type 1 from Human Epidermis: Virus Replication and Transmission Studies

摘要: Abstract For a better understanding of the pathogenetic events operative in cutaneous manifestations human immunodeficiency virus type 1 (HIV-1) disease, we investigated whether epidermal cells (EC) from HIV-1 -seronegative persons can be infected with and, vice versa, rescued epidermis HIV-1-infected individuals. In series three experiments, consistently found that exposure EC HIV-1-seronegative donors to led viral replication these as evidenced by detection p24 culture fluids. Because had been substantially enriched for Langerhans (LC) before being exposed HIV-1, it is reasonable assume CD1a + /CD4 /MHC class II antigen-presenting represented actual targets infection. This assumption further strengthened observation T cell-depleted cell suspensions histiocytosis (LCH) lesions could productively HIV-1. Conversely, co-culture sheets HIV-1-seropositive individuals mononuclear phagocytes (MNP) HIV- 1-seronegative resulted, after 3 5 weeks, 12 23 cases. Immunocytochemical analysis, using monoclonal antibody specific p24, revealed presence adherent MNP cocultures tested. addition, cellular DNA cultures showed strong signals when hybridized HIV-1-specific probe. The finding two isolates examined exhibited different restriction enzyme patterns indicates they are separate entities rather than contaminants. Transmission MNP, B- or T-cell lines resulted strongly positive case H9 cells, particles detected electron microscopy. Our results therefore suggest not only serve but also allow efficient and transmit various types hematopoietic lineage.