Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids

摘要: Abstract The growth of analytical methods for the detection nucleic acid from various biological samples reflects recent advances in biotechnology development especially areas genetic, infections and cancer diagnosis. target DNA is detected by hybridization techniques derived Southern's blotting. However such assays, based on use 32 P labelled probes, bring with them associated problems handling radioactive materials. In order to overcome these difficulties, a number chemiluminescent have recently been developed. These new, alternative probe labelling procedures are easy routine assays performed research laboratories as well medical applications, can reach level sensitivity found classical radiolabelling techniques. investigated include peroxydase, biotin 16-dUTP or digoxigenin 11-dUTP labelling. DNAs transferred onto nitrocellulose nylon membranes further fixed heat UV crosslinking. Specific finally revealed substrates. For all limit 10 aM (attomol) 561 bp DNA. probes peroxydase drops 1.0 present paper we shall compare several show that threshold vary much factor 20 method method. This first time label compared evaluated determine best protocol.