Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane.

摘要: Methyl coenzyme-M reductase A ( mcrA ) clone libraries were generated from microbial DNA extracted the rumen of cattle fed a roughage diet with and without supplementation antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c . 30%, resultant increase in propionate branched chain fatty acids. The revealed that Methanobrevibacter spp. dominant species identified. decrease incidence library bromochloromethane treatment was observed. In addition, more diverse methanogenic population representatives Methanococcales , Methanomicrobiales Methanosacinales orders observed for library. Sequence data these aided design an -targeted quantitative PCR (qPCR) assay. reduction production associated average 34% number Archaea when monitored this qPCR Dissociation curve analysis amplicons showed clear difference melting temperatures spp et al. (80–82 °C) all other methanongens (84–86 °C). intensity specific peak bromochloromethane-treated animals corresponded changes within libraries.