In silico characterization of SAMdC from Pokkali rice and its overexpression in transgenic tobacco

摘要: Polyamines help to mitigate salt stress in plants. SAMdC or S-adenosyl methionine decarboxylase is a crucial enzyme the polyamine biosynthetic pathway. Overexpressing samdc gene of Pokkali (a salt-tolerant Indica rice landrace) model plant tobacco through Agrobacterium-mediated transformation resulted plants capable withstanding high salinity (200 mM) stress. The transgenic maintained steady level higher polyamines and resisted ionic imbalance under Since appropriate binding substrate with essential for an enzymatic reaction, we performed molecular docking experiment get idea about its commonality two backdrop Japonica Arabidopsis. In silico characterization revealed that used same tobacco, from where introgressed it overexpressed.