Integrative analysis of transcriptional profile reveals LINC00052 as a suppressor of breast cancer cell migration.

摘要: Background Long-non-coding RNAs, a class of transcripts with lengths > 200 nt, play key roles in tumour progression. Previous reports revealed that LINC00052 (long intergenic non-coding RNA 00052) was strongly downregulated during breast cancer multicellular spheroids formation and suggested role cell migration oxidative metabolism. Objective To examine the function MCF-7 cells. Methods Loss-of-function studies were performed to evaluate on Microarray expression assays determine genes cellular functions modified after knockdown. Next, impact depletion respiration evaluated. Results 1,081 differentially expressed upon inhibition. Gene set enrichment analysis, Ontology Key Pathway Advisor analysis showed signalling networks related phosphorylation enriched. However, whereas knockdown cells marginal difference oxygen consumption rates when compared control cells, inhibition enhanced vitro vivo, as observed using Zebrafish embryo xenotransplant model. Conclusion Our data show modulates migration. Genome-wide microarray experiments suggest is affected by through cytoskeleton modulation Notch/β-catenin/NF-κB pathways.