Growth Hormone Receptor Gene is Essential for Chicken Mitochondrial Function In Vivo and In Vitro.
作者： Bowen Hu , Shuang Hu , Minmin Yang , Zhiying Liao , Dexiang Zhang
DOI: 10.3390/IJMS20071608
关键词:
摘要: The growth hormone receptor (GHR) gene is correlated with many phenotypic and physiological alternations in chicken, such as shorter shanks, lower body weight muscle mass loss. However, the role of GHR mitochondrial function remains unknown poultry. In this study, we assessed mitochondria sex-linked dwarf (SLD) chicken skeletal interfered expression DF-1 cells to investigate both vivo vitro. We found that key regulators biogenesis DNA (mtDNA)-encoded oxidative phosphorylation (OXPHOS) genes were downregulated accompanied by reduced enzymatic activity OXPHOS complexes SLD knockdown cells. Then, measuring membrane potential (ΔΨm), swelling, reactive oxygen species (ROS) production, malondialdehyde (MDA) levels, ATP levels respiratory control ratio (RCR), was impaired addition, also studied morphology structure transmission electron microscopy (TEM) MitoTracker staining. could reduce number alter Above all, demonstrated for first time essential
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