Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation.

摘要: A chromosomal DNA fragment which mediates Pap (pili associated with pyelonephritis) pili formation, mannose-resistant hemagglutination ( MRHA ) and binding to uroepithelial cells has been isolated from the uropathogenic Escherichia coli clinical isolate J96 , genetically studied. Analysis of polypeptides expressed by led detection a number ranging in mol. wt. 13 000 81 daltons. The gene order transcriptional orientation for four corresponding cistrons was: papB 19 500 papA structural pilus subunit), papC 28 papD ). Analyses lacZ- fusion located promoter upstream within cloned DNA. Transposon Tn5 insertions any these decreased or eliminated formation. transposon mutations were identified region distal that normal levels protein on cell surface form recognizable structures but did not agglutinate human erythrocytes adhere cells. This 15 000, 24 26 35 finding shows formation properties can be dissociated.