Smoke decreases reversible oxidations S-glutathionylation and S-nitrosylation in mice
作者： Ine Kuipers , Ken R Bracke , Guy G Brusselle , Emiel Fm Wouters , Niki L Reynaert
DOI: 10.3109/10715762.2011.647011
关键词:
摘要: Cigarette smoke causes irreversible oxidations in lungs, but its impact on reversible and physiologically relevant redox-dependent protein modifications remains to be investigated. Here the effect of cigarette exposure mice was investigated covalent binding glutathione thiols, known as S-glutathionylation (PSSG), which can reversed by glutaredoxins (Grx). Also, S-nitrosylation (PSNO) is modification thiols NO enzyme alcohol dehydrogenase (ADH) 5 examined. Both PSSG PSNO levels lung tissue were markedly decreased after 4 weeks exposure. This coincided with attenuated free thiol increased carbonylation. The expression NOX4, DHE sensitive oxidant production iNOS induced smoke, whereas Grx1 mRNA activity attenuated. Free GSH levels, ADH5 unaffected smoke. Taken together, decreases cysteine enhances These alterations are not associated differences some regulatory enzymes, likely result oxidative stress.
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