Selective Medium for Isolating Staphylococci

作者: Karl-Heinz Schleifer , Eberhard Krämer

DOI: 10.1016/S0172-5564(80)80008-X

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摘要: Summary A selective medium was developed for isolating coagulase-positive and coagulase-negative staphylococci. The agents employed were sodium azide, potassium thiocyanate, lithium chloride glycine. Addition of glycine is only necessary if high counts streptococci are expected. All type strains the various staphylococcal species other staphylococci tested revealed good growth on this new medium. S. intermedius exception since its recovery significantly lower than control However, normal possible at a azide concentration (15–20 mg/litre). Growth all micrococci bacteria such as bacilli, arthrobacters, lactobacilli, streptococci, Escherichia coli, Enterobacter cloacae Proteus mirabilis inhibited: these organisms do not grow or most pinpoint-sized colonies. egg yolk pork plasma to may provide basis distinguishing aureus from useful isolation but also represents routine method fur separation micrococci. great specificity could be demonstrated in detecting foods levels low 100/g.

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