“A novel therapeutic approach to corneal alkaline burn model by targeting Fidgetin-like 2, a microtubule regulator”

摘要: Purpose The purpose of this study was to determine the efficacy nanoparticle-encapsulated Fidgetin-like 2 (FL2) siRNA (FL2-NPsi), a novel therapeutic agent targeting FL2 gene, for treatment corneal alkaline chemical injury. Methods Eighty 12-week-old, male Sprague-Dawley rats were divided evenly into 8 groups: prednisolone, empty nanoparticles, control-NPsi (1 µM, 10 and 20 µM) FL2-NPsi µM). An burn induced onto cornea each rat, which then treated 14 days according group assignment. Clinical, histopathologic, immunohistochemical analyses conducted assess wound healing. FL2-NPsi-mediated knockdown confirmed by in vitro quantitative polymerase chain reaction (qPCR). Toxicity assays performed apoptosis (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling [TUNEL] assay) nerve damage (whole mount immunochemical staining). Statistical using Student's t-test ANOVA. Results Compared with controls, FL2-NPsi-treated groups demonstrated enhanced healing, µM demonstrating maximum rates re-epithelialization as assessed ImageJ software, transparency, improved stromal organization on histology. Immunohistochemical analysis vascular endothelial cells, macrophages, neutrophils did not show significant differences between groups. found be toxic nerves or induce (p = 0.917). Conclusions Dose-response studies both efficacious rat model. may offer injuries. Translational relevance Basic cell biology findings about microtubule cytoskeleton used design enhance migration, highlighting promise microtubules regulate