Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus

摘要: Cohesin-mediated sister chromatid cohesion is essential for chromosome segregation and post-replicative DNA repair. In addition, evidence from model organisms human genetics suggests that cohesin involved in the control of gene expression. This non-canonical role has recently been rationalized by findings mammalian complexes are recruited to a subset DNase I hypersensitive sites conserved noncoding sequences DNA-binding protein CTCF. CTCF functions at insulators (which interactions between enhancers promoters) boundary elements demarcate regions distinct chromatin structure), contributes its enhancer-blocking activity. The underlying mechanisms remain unknown, full spectrum remains be determined. Here we show forms topological mechanistic basis cell-type-specific long-range chromosomal cis developmentally regulated cytokine locus IFNG. Hence, ability constrain topology used not only purpose cohesion, but also dynamically define spatial conformation specific loci. new aspect function probably important normal development disease.