Implementation of molecular detection techniques in the field of veterinary virology

摘要: This thesis deals with the molecular diagnosis of infectious diseases concerning animal and human health: in particular, notifiable to World Organization for Animal Health, OIE (the vesicular complex avian influenza), as well Hepatitis E, representing emerging zoonotic aspects. With worldwide introduction use polymerase chain reaction (PCR) methodologies, detection different pathogens improved significantly - however, these systems have weak points well. The parallel screening more than a few is not resolved and, general, multiplexing capacity most methods used this area insufficient. In case causing similar symptoms (like complex, involving Foot-and-Mouth Disease (FMD), Swine Vesicular (SVD) Stomatitis (VS), immediate differential essential, only regarding multiplexing, but also because high economic risks strict legislations. Subtyping influenza viruses broad extensive task it needs differentiate 16 hemagglutinin 9 neuraminidase types their variants. padlock probes, studies, seem be optimal fulfill requirements provide novel, high-throughput tools influenza. general subtyping E Virus (HEV) an important complicated today, virus shows potential by endemics humans persisting infections species. Thus, there need sensitive specific identification HEV frame work, highly diagnostic assay was developed, based on two real-time PCR methods. addition, genotyping system constructed using simple quick ligation-based technique.