MicroRNA-331-3p inhibits epithelial-mesenchymal transition by targeting ErbB2 and VAV2 through the Rac1/PAK1/β-catenin axis in non-small-cell lung cancer.
作者： Xizhe Li , Jiali Zhu , Yuanqi Liu , Chaojun Duan , Ruimin Chang
DOI: 10.1111/CAS.14014
关键词:
摘要: MicroRNAs have been reported to play critical roles in the regulation of non-small-cell cancer (NSCLC) development, but role microRNA (miR)-331-3p NSCLC is still unclear. In this study, expression levels miR-331-3p tumor tissues and adjacent normal were examined by quantitative RT-PCR, relationship between patient clinicopathological characteristics was analyzed. The effects on epithelial-mesenchymal transition (EMT), migration, metastasis cells determined vitro vivo. Direct functional targets identified luciferase reporter assay, western blot immunohistochemical staining, rescue assay. downstream pathway regulated immunofluorescence, immunoprecipitation, Rac1 activity examination. Our results showed that significantly downregulated correlated with characteristics, could be an independent prognostic marker for patients. Furthermore, suppressed EMT, migration Both ErbB2 VAV2 direct miR-331-3p. activities Rac1, PAK1, β-catenin through targeting. These indicated suppresses migratory capacity, metastatic ability targeting Rac1/PAK1/β-catenin axis NSCLC.
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