Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania.

摘要: Brucellosis is a zoonotic disease of importance to both public health and the livestock industry. The likely be endemic in Tanzania little reported on molecular characterization Brucella species pastoral settings. This study aimed at characterizing (targeting genus Brucella) infecting humans, cattle goat Kagera region (Ngara Karagwe districts) using real-time PCR, PCR amplification 16S rRNA genes Sanger sequencing. spp. were detected 47 samples (19 sera 28 milk) out 125 (77 sera, 35 milk 13 aborted materials) PCR. All materials (13 samples) negative Out positive (28 19 sera), 20 (10 10 sera) showed an expected gene product. Sequence analysis blasting confirmed presence areas region. from phylogenetically grouped two clades three branches all closer B. melitensis, abortus suis USA, Sudan Iran. However, they distinct other isolated also New Zealand, Germany Egypt. was based distance between geographical regions which data (nucleotides sequences sequencing) for phylogeny reconstruction obtained. first report identified sequencing East Central Africa. A vaccination program re-inforced with high index diagnosis needed eradicate brucellosis animals minimize suffering infections humans Tanzania.