Comprehensive metabolomic study of platelets reveals the expression of discrete metabolic phenotypes during storage.

摘要: Background Platelet (PLT) concentrates are routinely stored for 5 to 7 days. During storage they exhibit what has been termed PLT lesion (PSL), which is evident by a loss of hemostatic function when transfused into patients. The overall goal this study was obtain comprehensive data set describing metabolism during storage. Study Design and Methods The experimental approach adopted achieve combined series standard assays monitor the quality PLTs deep-coverage metabolomics using liquid chromatography coupled with mass spectrometry performed on both extracellular intracellular environments. we measured 174 different variables in 6 units, collected apheresis. Samples were at eight time points resulting containing more than 8000 measurements. Results Stored did not undergo monotonic decay, but experienced systematic changes reflected three discrete metabolic phenotypes: first (Days 0-3) associated active glycolysis, pentose phosphate pathway, glutathione down regulation tricarboxylic acid (TCA) cycle. second 4-6) TCA cycle as well increased purine metabolism. A third phenotype less clinical relevance 7-10) faster decay cellular metabolism. Conclusion PSL linear metabolism, rather successive shifts. These findings may give new insight mechanisms underlying PSL encourage deployment systems biology methods PSL.