Process for producing modified inorganic polymers, their use in producing polynucleotides, and a reagent useful in these processes
作者： Marvin H. Caruthers , Mark D. Matteucci
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摘要: Modified inorganic polymers represented by the formula wherein Ⓟ is an polymer linked to 3' or 5' -0- of nucleoside through a base hydrolyzable covalent bond; R H blocking group; hydrocarbyl radical containing up 10 carbons; each B deoxynucleoside base; and A OR. Said are chemically bound nucleoside. The chemical bonding moiety means reactive groups on which react with molecule. Such modified supports useful in stepwise addition nucleosides oligonucleotides original support series process steps. starting blocked either 3'- 5'-OH coupled unblocked hydroxy group bythe coupling agent. Furthermore automated apparatus for producing silica gel example shown.
lens.org 参考文章(5)
Pietro Tundo, Paolo Venturello, Synthesis, catalytic activity, and behavior of phase-transfer catalysts supported on silica gel. Strong influence of substrate adsorption on the polar polymeric matrix on the efficiency of the immobilized phosphonium salts Journal of the American Chemical Society. ,vol. 101, pp. 6606- 6613 ,(1979) , 10.1021/JA00516A021
M.D. Matteucci, M.H. Caruthers, The synthesis of oligodeoxyprimidines on a polymer support Tetrahedron Letters. ,vol. 21, pp. 719- 722 ,(1980) , 10.1016/S0040-4039(00)71455-9
H. Köster, K. Heyns, Polymer support oligonucleotide synthesis VII use of Sephadex LH 20 Tetrahedron Letters. ,vol. 13, pp. 1531- 1534 ,(1972) , 10.1016/S0040-4039(01)84678-5
Gurdip S. Bajwa, Wesley G. Bentrude, Thymidine nucleoside 3′,5′-cyclic phosphoramidites and phosphites. Configuration at phosphorus in trivalent and pentavalent cyclic nucleotides by 31P and 13C nmr. Tetrahedron Letters. ,vol. 19, pp. 421- 424 ,(1978) , 10.1016/S0040-4039(01)91444-3
Serge L Beaucage, Marvin H Caruthers, Phosphoramidite compounds and their use in producing oligonucleotides ,(1982)