MLL-ENL causes a reversible and myc-dependent block of myelomonocytic cell differentiation.
作者： Robert Karl Slany , Marco Birke , María-Paz García-Cuéllar , Silke Schreiner , Johann Greil
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摘要: The translocation t(11;19) is a recurrent feature of subgroup acute leukemiasoccurring in infants. This event fuses the genes MLL and ENL creates leukemogenic oncoprotein MLL-ENL. We studied effect retroviral MLL-ENL expression primary mouse hematopoietic cells show here that requires Myc to establish reversible differentiation arrest myelomonocytic precursor population. MLL-ENL-transduced proliferated as immature myeloid presence interleukin 3. addition granulocyte colony-stimulating factor reversed maturation block set by induced development mature granulocytes macrophages accompanied growth arrest. Gene analysis indicated down-regulation proto-oncogene c-myc several target during factor-mediated differentiation. role transformation pathway was tested modulating effective protein concentrations transduced cells. Cotransduction dominant-negative neutralized precluded transformation. In contrast, constitutive cooperated with caused cell population an irreversible
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