A novel spectrofluorimetric method for the determination of calcitonin in ampules through derivatization with fluorescamine

摘要: In this study, a simple, sensitive and selective spectroflourimetric method has been developed for the determination of salmon calcitonin (sCT) in ampules. The is based on reaction between sCT fluorescamine at pH 8.5 borate buffer, resulting highly fluorescent derivative. Fluorescence derivatized solutions was measured by setting excitation emission monochromators slit widths to 390, 484 10 nm, respectively. Sevaral derivatization parameters were optimized. A calibration graph constructed using standard range 0.5-6.0 µg/mL. Limit detection limit quantification values determined be 0.124 0.372 µg/mL, proposed successfully applied commercially available High recovery (101.0-102.0 %), low relative deviation (RSD %) value (5.3-5.4) proved accuracy precision method. An isocratic reversed-phase high-performance liquid chromatographic (HPLC) method, as reference, also sCT. Nucleosil® C18 column (250 mm × 4.6 i.d., µm particle size, 120 pore size) used detector set 210 nm. Statistical comparison results two methods showed clearly that there no significant difference them.