Novel genes and functional relationships in the adult mouse gastrointestinal tract identified by microarray analysis

摘要: Abstract Background & Aims: A genome-level understanding of the molecular basis segmental gene expression along anterior-posterior (A-P) axis mammalian gastrointestinal (GI) tract is lacking. We hypothesized that functional patterning A-P GI could be defined at level by analyzing profiles large numbers genes. Methods: Incyte GEM1 microarrays containing 8638 complementary DNAs (cDNAs) were used to define in adult mouse stomach, duodenum, jejunum, ileum, cecum, proximal colon, and distal colon. Highly expressed cDNAs classified based on patterns protein function. Results: 571 2-fold higher than reference least 1 tissue. Most these genes displayed sharp boundaries, majority which anatomically locations. Boundaries particularly striking for encoding proteins function intermediary metabolism, transport, cell-cell communication. Genes with distinctive compared human genomic sequence promoter analysis discovery. Conclusions: The organs (stomach, small intestine, colon) can distinguished a profiles. However, distinctions between various regions intestine colon are much less striking. have identified novel not previously known tract. Identification coordinately regulated provides new insights discovery relevant development, differentiation, function, disease. GASTROENTEROLOGY 2002;122:1467-1482