Transfer of contaminants in adeno-associated virus vector stocks can mimic transduction and lead to artifactual results.
作者： Ian E. Alexander , David W. Russell , A. Dusty Miller
DOI: 10.1089/HUM.1997.8.16-1911
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摘要: ABSTRACT The potential of adeno-associated virus (AAV) vectors for gene transfer and therapy applications is currently being intensively investigated. Although much progress has been made in defining AAV vector biology, inconsistencies remain the literature regarding efficiency transduction various cell types. In course exploring these differences, we have identified a problem associated with use stocks that results overestimation efficiencies. We show here biologically active vector-encoded proteins can contaminate stocks, especially lysate preparations not further purified, be transferred virion-independent manner to target cells, phenomenon called pseudotransduction. This observation significant because impure widely employed literature, demonstrate this occur commonly used reporter such as β-galact...
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