Threshold effects on the lectin-mediated aggregation of synthetic glycolipid-containing liposomes.

摘要: Cholesterol analogs containing sugar residues linked by spacer groups to the cholesterol O can be incorporated into egg yolk lecithin small unilamellar liposomes. The synthetic glycolipid distribute evenly on both sides of bilayer. These liposomes are aggregated appropriate lectin. For example, when residue is a beta-galactoside ricin and it an alpha-mannoside they Con A. lectin-mediated aggregation these reversed addition sugar. rates but not extents highly sensitive amount incorporated. Below approximately 5% incorporation rate exceedingly slow, whereas above this level rapid proceeds. At all concentrations studied glycolipids in unimodal fashion so that observed threshold effects cannot based possible differences manner which at different concentrations. This conclusion 1) studies with galactose oxidase show percentage oxidation liposome prepared from galactosyl-containing independent concentration, 2) mixed shown behave independently. importance critical density membrane-bound receptors order for occur discussed.