Microstructured liposome subunit vaccines reduce lung inflammation and bacterial load after Mycobacterium tuberculosis infection

摘要: Abstract Background Tuberculosis is a disease affecting millions of people throughout the world. One main problems in controlling low efficacy Bacillus Calmette–Guerin (BCG) vaccine protecting young adults. The development new vaccines that induce long-lasting immune response or stimulate immunity induced by BCG may improve control tuberculosis. Methods use microstructured liposomes containing HspX, with without MPL CpG DNA adjuvants, as for tuberculosis was evaluated. HspX-specific humoral and cellular responses to different formulations were compared. Results All liposome microparticles HspX immunogenic. Vaccines formulated strongest responses, mainly inducing interferon-γ tumor necrosis factor-α expression both CD4 + CD8 T cells. T-cell activation specific responses. When evaluated protective against Mycobacterium challenge, L-HspX L-HspX-CPG reduced lung inflammatory lesions bacterial load. Conclusion We have thus demonstrated, first time, an adjuvant delivery system formulation