RIBOFLAVIN TRANSPORT IN YEASTS AND ITS REGULATION

摘要: Publisher Summary This chapter describes some properties of active riboflavin (RF) transport systems that do not operate in wild-type strains Pichia guilliermondii (P. guilliermondii) and discusses the detection RF excretase activity this species. permease does promote glucose into yeast cell, for inhibition uptake by glucose, penetration sugar across membrane is necessary. A study regulation synthesis showed flavins themselves did regulate formation system. The genetic analysis 139 mutants P. capable constitutive revealed two dominant one semidominant mutant; remaining 136 mutants, phenotype was recessive. complementation recessive classes. Mutants first class were designated as rfp80 those second rfp81. possesses system excretion from cell excretase. system, similar to permeases, characterized stringent substrate specificity; its depends on temperature pH medium. Inhibitors energy metabolism depressed while sugars other carbon sources significantly increased rate process. Thus, exit cells accomplished a specific excretion.