Opisthorchis viverrini: effect of praziquantel on the adult tegument.

摘要: Ultrastructural changes of the tegument adult liver flukes, Opisthorchis viverrini, after in vitro incubation Minimal Essential Medium containing 0, 0.1, 1.0 and 10.0 micrograms/ml anthelminthic praziquantel for 5, 15, 30, 45 60 minutes were investigated by scanning (SEM) transmission (TEM) electron microscopy. SEM observations showed that surface damage was composed blebbing due to swelling microvilli, followed later disruption these structures form lesions caused erosion desquamation surface. Sensory papillae, contrast, appeared relatively unaffected. The could be observed at all doses but extent increased with increasing duration concentration drug. ventral as well dorsal surfaces exhibited similar change, whereas anterior part tended damaged less than posterior part. Under TEM observations, earliest sign depolymerization microtrabecular network scattered foci, which resulted formation non-membrane-bound vacuoles under microvilli. basal infoldings also became dilated, some turned into membrane-bound zone. Subsequently, microvilli enlarged, eventually formed blebs rupture lesion spots SEM. Finally, regions broke down, creating various sizes throughout tegument, leading its total disintegration detachment. sequence morphological generally doses; however, occurred faster higher longer times. In addition, durations myofilaments most muscle cells depolymerized, while microtubules unchanged Therefore, it is possible praziquantel, through induction Ca2+ influx, causes leads vacuolization, swelling, blebbing, detachment breakdown cells.