Effects of aspect ratios of stem cells on lineage commitments with and without induction media.
作者： Xiang Yao , Rong Peng , Jiandong Ding
DOI: 10.1016/J.BIOMATERIALS.2012.10.052
关键词:
摘要: The present study is aimed to examine the shape effect on lineage commitment of stem cells in growth medium free external chemical induction factors. Aspect ratios (ARs) were controlled by micropatterns with cell-adhesive microislands AR 1, 2 and 8 potent nonfouling background poly(ethylene glycol) hydrogels, single well shaped for 19 days. Mesenchymal (MSCs) derived from rat bone marrow cultured osteogenic medium, adipogenic mixed coinduction also medium; alkaline phosphatase (ALP) oil droplets employed as indicators osteoblasts adipocytes, respectively. Those observed all three media early day 7, at a longer culture time till 13. While significant monotonic decrease adipogenesis was increase AR, non-monotonic change osteogenesis found optimal about 2. relative gene expressions further verified above findings. As result, cell itself an inherent cue regulate differentiation, let alone or without Such disappeared upon addition microfilament inhibitor cytochalasin D Rho-associated protein kinase (ROCK) Y-27632. So, formation cytoskeleton necessary effect, ROCK-pathway-related tension responsible even medium.
els-cdn.com参考文章(45)
Ce Yan, Jianguo Sun, Jiandong Ding, Critical areas of cell adhesion on micropatterned surfaces Biomaterials. ,vol. 32, pp. 3931- 3938 ,(2011) , 10.1016/J.BIOMATERIALS.2011.01.078
Rong Peng, Xiang Yao, Bin Cao, Jian Tang, Jiandong Ding, The effect of culture conditions on the adipogenic and osteogenic inductions of mesenchymal stem cells on micropatterned surfaces Biomaterials. ,vol. 33, pp. 6008- 6019 ,(2012) , 10.1016/J.BIOMATERIALS.2012.05.010
Rong Peng, Xiang Yao, Jiandong Ding, Effect of cell anisotropy on differentiation of stem cells on micropatterned surfaces through the controlled single cell adhesion. Biomaterials. ,vol. 32, pp. 8048- 8057 ,(2011) , 10.1016/J.BIOMATERIALS.2011.07.035
Wei Song, Naoki Kawazoe, Guoping Chen, Dependence of spreading and differentiation of mesenchymal stem cells on micropatterned surface area Journal of Nanomaterials. ,vol. 2011, pp. 6- ,(2011) , 10.1155/2011/265251
Neelam Jaiswal, Stephen E. Haynesworth, Arnold I. Caplan, Scott P. Bruder, Osteogenic differentiation of purified, culture‐expanded human mesenchymal stem cells in vitro Journal of Cellular Biochemistry. ,vol. 64, pp. 295- 312 ,(1997) , 10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I
Zhen Pan, Ce Yan, Rong Peng, Yingchun Zhao, Yao He, Jiandong Ding, Control of cell nucleus shapes via micropillar patterns. Biomaterials. ,vol. 33, pp. 1730- 1735 ,(2012) , 10.1016/J.BIOMATERIALS.2011.11.023
R Singhvi, A Kumar, G. Lopez, G. Stephanopoulos, D. Wang, G. Whitesides, D. Ingber, Engineering Cell Shape and Function. Science. ,vol. 264, pp. 696- 698 ,(1994) , 10.1126/SCIENCE.8171320
Kun Ma, Filip Laco, Seeram Ramakrishna, Susan Liao, Casey K. Chan, Differentiation of bone marrow-derived mesenchymal stem cells into multi-layered epidermis-like cells in 3D organotypic coculture. Biomaterials. ,vol. 30, pp. 3251- 3258 ,(2009) , 10.1016/J.BIOMATERIALS.2009.02.025
Jinghuan Huang, Jiandong Ding, Nanostructured interfaces with RGD arrays to control cell–matrix interaction Soft Matter. ,vol. 6, pp. 3395- 3401 ,(2010) , 10.1039/B927168F
Daniel Aydin, Ilia Louban, Nadine Perschmann, Jacques Blümmel, Theobald Lohmüller, Elisabetta Ada Cavalcanti-Adam, Tobias L Haas, Henning Walczak, Horst Kessler, Roberto Fiammengo, Joachim P Spatz, None, Polymeric Substrates with Tunable Elasticity and Nanoscopically Controlled Biomolecule Presentation Langmuir. ,vol. 26, pp. 15472- 15480 ,(2010) , 10.1021/LA103065X