Developmental expression of μ and δ opioid receptors in the rat brainstem: evidence for a postnatal switch in μ isoform expression
作者： Bronwyn M Kivell , Darren J Day , Fiona J McDonald , John H Miller
DOI: 10.1016/J.DEVBRAINRES.2003.12.002
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摘要: Abstract Opioid receptors are expressed in the brain during fetal and postnatal development, expression patterns vary with developmental age. To investigate role of opioids immunoblotting immunohistochemical techniques were used to determine μ (MOR) δ (DOR) opioid receptor levels regional distributions fetal, early adult rat brainstem. Two immunoreactive bands seen on Western blots brainstem lysates for both MOR (50 70 kDa) DOR (30 60 kDa). The isoforms changed dramatically between 6 15 days after birth. Total protein was at low animals 50-kDa band predominating. then increased older 70-kDa isoform became dominant. showed opposite pattern, being high neonatal juvenile adult. A switch not evident our study. In general, developing comparable those reported literature, localized same areas where high. It concluded that developmentally regulated rat, ratio switches postnatally from a fetal–neonatal pattern juvenile–adult generally regions E16
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sci-hub.se 参考文章(51)
U Arvidsson, RJ Dado, M Riedl, JH Lee, PY Law, HH Loh, R Elde, MW Wessendorf, delta-Opioid receptor immunoreactivity: distribution in brainstem and spinal cord, and relationship to biogenic amines and enkephalin The Journal of Neuroscience. ,vol. 15, pp. 1215- 1235 ,(1995) , 10.1523/JNEUROSCI.15-02-01215.1995
U Arvidsson, M Riedl, S Chakrabarti, JH Lee, AH Nakano, RJ Dado, HH Loh, PY Law, MW Wessendorf, R Elde, Distribution and targeting of a mu-opioid receptor (MOR1) in brain and spinal cord The Journal of Neuroscience. ,vol. 15, pp. 3328- 3341 ,(1995) , 10.1523/JNEUROSCI.15-05-03328.1995
J L Juarros, P Sanchez-Blazquez, J Garzon, M A Castro, Antibodies to the cloned mu-opioid receptor detect various molecular weight forms in areas of mouse brain. Molecular Pharmacology. ,vol. 47, pp. 738- 744 ,(1995)
JW Spain, BL Roth, CJ Coscia, Differential ontogeny of multiple opioid receptors (mu, delta, and kappa) The Journal of Neuroscience. ,vol. 5, pp. 584- 588 ,(1985) , 10.1523/JNEUROSCI.05-03-00584.1985
Mariana M. Belcheva, Elena G. Ignatova, Eric C. Young, Carmine J. Coscia, Agonist-induced desensitization and down-regulation of delta opioid receptors alter the levels of their 125I-beta-endorphin cross-linked products in subcellular fractions from NG108-15 cells. Biochemistry. ,vol. 35, pp. 14818- 14824 ,(1996) , 10.1021/BI961579+
R. R. Goodman, S. H. Snyder, M. J. Kuhar, W. S. Young, Differentiation of delta and mu opiate receptor localizations by light microscopic autoradiography Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 6239- 6243 ,(1980) , 10.1073/PNAS.77.10.6239
Atalie S. Colman, John H. Miller, Modulation of breathing by μ1 and μ2 opioid receptor stimulation in neonatal and adult rats Respiration Physiology. ,vol. 127, pp. 157- 172 ,(2001) , 10.1016/S0034-5687(01)00240-7
Wojciech T. Bem, S. Jim Yeung, Mariana Belcheva, Jacob Barg, Carmine J. Coscia, Age‐Dependent Changes in the Subcellular Distribution of Rat Brain μ‐Opioid Receptors and GTP Binding Regulatory Proteins Journal of Neurochemistry. ,vol. 57, pp. 1470- 1477 ,(1991) , 10.1111/J.1471-4159.1991.TB06340.X
Immanuela Ravé Moss, André Laferrière, Central neuropeptide systems and respiratory control during development Respiratory Physiology & Neurobiology. ,vol. 131, pp. 15- 27 ,(2002) , 10.1016/S1569-9048(02)00034-4
Daniel J.J. Carr, Brian DeCosta, Arthur E. Jacobson, Kenneth L. Bost, Kenner C. Rice, J.Edwin Blalock, Immunoaffinity-purified opiate receptor specifically binds the δ-class opiate receptor ligand, cis-(+)-3-methylfentanylisothiocyanate, SUPERFIT FEBS Letters. ,vol. 224, pp. 272- 276 ,(1987) , 10.1016/0014-5793(87)80468-4