An experimental design methodology applied to the enantioseparation of a non-steroidal anti-inflammatory drug candidate.

摘要: An experimental design methodology has been applied to the enantioseparation of a new synthesized aryl propionic acid pharmaceutical interest, namely 2-[(4'-benzoyloxy-2'-hydroxy)phenyl-propionic acid] (DF-1770y) by chiral capillary zone electrophoresis (CCZE). The separation studied compound achieved employing vancomycin as selector. partial filling-counter current method used in order avoid presence absorbing selector path length detector and increase sensitivity. A central composite employed optimize conditions for fast enantiomers acid. Critical parameters such concentration, pH temperature have evaluate how they affected responses resolution migration times. desirability function approach find best compromise between different responses. proposed CCZE provided baseline investigated drug. Britton-Robinson buffer at 6.4 supplemented with 7 mM 22 degrees C -20 kV were optimum allowing achieve highest enantioresolution DF-1770y less than 8.5 min.