Antidiabetic, antihyperlipidemic and antioxidant effects of the flavonoid rich fraction of Pilea microphylla (L.) in high fat diet/streptozotocin-induced diabetes in mice.

摘要: The present study describes the antidiabetic effect of flavonoid rich fraction Pilea microphylla (PM1). HPLC characterization PM1 revealed presence polyphenols viz., chlorogenic acid, rutin, luteolin-7-O-glucoside, isorhoifolin, apigenin-7-O-glucoside, and quercetin. inhibited dipeptidyl peptidase IV (DPP-IV) in vitro with an IC50 520.4 ± 15.4 μg/ml. PM1, at doses 300, 600 900 mg/kg i.p., also produced dose-dependent mean percent reductions 9.9, 30.6 41.0 glucose excursion (AUC0–120 min) respectively lean mice. However, even highest dose did not alter normoglycemic condition. 100 mg/kg/day, i.p. for 28 days significant (p < 0.05) reduction body weight, plasma (PG), triglycerides (TG) total cholesterol (TC) content high-fat streptozotocin-induced diabetic improved oral tolerance significantly percentage 48.0% enhanced endogenous antioxidant status mice liver compared to control. preserved islet architecture prevented hypertrophy hepatocytes as evident from histopathology pancreas liver. show any detectable hematological toxicity therapeutic doses. In conclusion, exhibits possibly by inhibiting DPP-IV improving levels high fat diet/streptozotocin (HFD/STZ)