KOC (K homology domain containing protein overexpressed in cancer): a novel molecular marker that distinguishes between benign and malignant lesions of the pancreas.

摘要: KOC (K homology domain containing protein overexpressed in cancer) is a novel oncofetal RNA-binding highly expressed pancreatic carcinomas. Recently, Corixa Corporation developed monoclonal antibody specific for that can be used with standard immunohistochemical techniques. The purposes of this study were 1) to assess mRNA expression carcinoma, 2) determine the pattern immunoexpression among benign, borderline, and malignant epithelial lesions, 3) evaluate utility distinguishing between these entities. was isolated from fresh tissues (19 carcinomas, 2 normal pancreas, 1 chronic pancreatitis) amplified using RT-PCR Fifteen 19 (79%) carcinomas relative non-neoplastic tissue samples increased progressively tumor stage: mean copy number transcripts 1.5, 11.1, 31, 28 stage I, II, III, IV respectively, compared 0.9 pancreatitis, respectively. Immunostains performed on 50 surgical resection specimens (38 invasive adenocarcinomas, 3 intraductal papillary-mucinous neoplasms, mucinous cystic 7 pancreatitis). staining present 37 38 (97%) carcinomas: reaction moderate or strong 36 (94%) >50% cells 35 (92%) cases. Severe dysplasia ductal epithelium, foci papillary cystadenocarcinoma, grade intraepithelial neoplasia (PanIN3) showed 15 cases, whereas mild (intraductal neoplasms adenoma and/or dysplasia, PanIN1, PanIN2) uniformly negative marker 25 22 In weak background acini 12 (24%) cases but easily distinguishable type identified neoplastic benign ducts ductules all Four (11%) resections pancreatitis as well 31 peritumoral <10% ductules. We conclude sensitive high-grade dysplastic lesions epithelium. Therefore, immunostains directed against may diagnostic evaluation particularly when biopsy material limited.