Graphene Oxide-Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy.

摘要: The use of graphene to target and eliminate cancer stem cells (CSCs) is an alternative approach conventional chemotherapy. We show the biomolecule-mediated synthesis reduced oxide–silver nanoparticle nanocomposites (rGO–Ag) using R-phycoerythrin (RPE); resulting RPE–rGO–Ag was evaluated in human ovarian (OvCSCs). synthesized nanocomposite (referred as rGO–Ag) characterized various analytical techniques. rGO–Ag showed significant toxicity towards both OvCSCs. After 3 weeks incubating OvCSCs with rGO–Ag, number A2780 ALDH+CD133+ colonies significantly reduced. toxic cell viability by mediating generation reactive oxygen species, leakage lactate dehydrogenase, mitochondrial membrane potential, enhanced expression apoptotic genes, leading dysfunction possibly triggering apoptosis. cytotoxic potential highly tumorigenic cells. combination salinomycin induced 5-fold higher levels apoptosis than each treatment alone. A at very low concentrations may be suitable for selectively killing sensitizing tumor a novel nano-therapeutic molecule specific targeting eliminating CSCs. This study highlights targeted therapy tumor-initiating