Two GEO MicroRNA Expression Profile Based High-Throughput Screen to Identify MicroRNA-31-3p Regulating Growth of Medullary Thyroid Carcinoma Cell by Targeting RASA2.

摘要: BACKGROUND Medullary thyroid carcinoma (MTC), a rare type of cancer, is big challenge in clinical treatment. However, the pathogenesis MTC remains poorly understand. MicroRNAs (miRNAs) were previously demonstrated to be involved MTC, however, roles majority miRNAs are still undetermined. MATERIAL AND METHODS Two GEO miRNA expression profiles (GSE40807, GSE97070) downloaded, and differentially expressed (DEmiRNAs) GSE40807 GSE97070 analyzed by bioinformatics methods. Expressions detected quantitative real-time polymerase chain reaction; cell proliferation was examined through Cell Counting Kit-8, colony formation vivo tumor growth assays; interaction between mRNA verified dual-luciferase reporter assay; functional analysis target genes performed using Database for Annotation, Visualization Integrated Discovery (DAVID, www.david.ncifcrf.gov) software. RESULTS Ten identified dysregulated both datasets, miR-31-3p showed highest change fold (Log change=-3.460625 Log change=-0.07084374 GSE97070). MiR-31-3p significantly downregulated low poor prognosis relative high (P<0.05). Functionally, inhibited vitro vivo. Functional also that numerous biochemical processes pathways, which Ras signaling pathway selected further study. RASA2, overexpressed negatively regulated miR-31-3p. In addition, we found knockdown RASA2 proliferation. CONCLUSIONS Reduced level might play key role tumorigenesis targeting critical especially pathway.