Intracellular processing of poly(ethylene imine)/ribozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments.
作者： Thomas Merdan , Klaus Kunath , Dagmar Fischer , Jindrich Kopecek , Thomas Kissel
关键词:
摘要: Purpose. Critical steps in the subcellular processing of poly(ethylene imine)/nucleic acid complexes, especially endosomal/lysosomal escape, were visualized by using living cell confocal laser scanning microscopy (CSLM) to obtain an insight into their mechanism.
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