Flow cytometric analysis reveals the high levels of platelet activation parameters in circulation of multiple sclerosis patients

摘要: The epidemiological studies confirm an increased risk of cardiovascular disease in multiple sclerosis, especially prothrombotic events directly associated with abnormal platelet activity. aim our study was to investigate the level blood activation circulation patients chronic phase sclerosis (SP MS) and their reactivity response typical platelets' physiological agonists. We examined 85 SP MS diagnosed according revised McDonald's criteria 50 healthy volunteers as a control group. were assessed using flow cytometry analysis following: P-selectin expression (CD62P), GP IIb/IIIa complex (PAC-1 binding), formation microparticles (PMPs) aggregates (PA) agonist-stimulated (ADP, collagen) unstimulated whole samples. Furthermore, we measured soluble (sP-selectin) plasma ELISA method, evaluate vivo activation, both subjects. found statistically significant increase expression, PMPs PA, well platelets samples from comparison also determined higher sP-selectin subjects than Based on obtained results, might conclude that during course are chronically activated display hyperreactivity agonists, such ADP or collagen.