The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat shock response.

摘要: The PKC1 gene of budding yeast encodes a homolog the alpha, beta, and gamma isoforms mammalian PKC that is proposed to regulate MAPK-activation pathway. Mutants in this pathway undergo cell lysis resulting from deficiency wall construction when they attempt grow at elevated temperatures. We show PKC1-regulated important for induced thermotolerance MPK1 protein kinase (the MAPK pathway) strongly activated by mild heat shock. This activation sustained during growth high temperature dependent on function components upstream MPK1, including PKC1. Expression genes under control known shock-inducible promoter elements (HSEs STREs) was not compromised mutants, indicating mediates novel aspect shock response. propose heat-induced signal generated response weakness created thermal stress, perhaps as result increased membrane fluidity. Evidence presented mechanism which detects measuring stretch plasma membrane.