The Auxin Receptor: Structure and Distribution

摘要: A range of antibodies has been developed to probe the structure and function maize auxin receptor. The epitopes recognised by a number polyclonal sera our monoclonal have mapped with aid an epitope mapping kit. recognise three principal epitopes, two which are conserved in other species. Two monoclonals endoplasmic reticulum retention sequence (-KDEL) at C-terminus, another region close N-terminus. We therefore markers for specific parts receptor, we using these identify functionally active protein. Additionally, against synthetic peptide. This peptide was identified from published receptor as being likely contribute binding site. anti-peptide on immunoblots potent agonist activity tobacco leaf mesophyll protoplasts, eliciting characteristic hyperpolarisation plasma membrane. By contrast, antisera native act antagonists. means that least part same experiments, others impermeant auxin-protein conjugates, show is outside However, bulk protein accordance its KDEL targeting this dual distribution will be discussed.