Determination of clozapine and its metabolite, norclozapine in various biological matrices using high-performance liquid chromatography.
作者： Micheal Rosland , Priscilla Szeto , Ric Procyshyn , Alasdair M. Barr , Kishor M. Wasan
DOI: 10.1080/03639040701484338
关键词:
摘要: Purpose. To develop and validate an HPLC method for the quantitation of clozapine its metabolite, norclozapine in human plasma, rat various plasma lipoprotein fractions. Methods. Using liquid-liquid extraction, clozapine, are extracted from biological matrix with MTBE. After concentration, residue was reconstituted 1mM HCl injected on to a C6 Phenyl column (3μm, 2.0×150 mm). Mobile phase 10mM Ammonium Acetate, pH 5—Acetonitrile—Methanol (5:3:2, v/v/v). Loxapine served as internal standard. Absorbance measured at 254 nm. Results. Quantitation limits 100 ng/mL 50 ng/mL, respectively. Recovery both near 100%. Percent accuracy intraday variability TRL, HDL, LDL, LPDP fraction between 92 113% analytes. Intraday precision same matrices less than 9% CV accura...
sci-hub.se 参考文章(12)
Kishor M. Wasan, Shawn M. Cassidy, Manisha Ramaswamy, Allison Kennedy, Frank W. Strobel, Samson P. Ng, Tak‐Yee Lee, A Comparison of Step-Gradient and Sequential Density Ultracentrifugation and the Use of Lipoprotein Deficient Plasma Controls in Determining the Plasma Lipoprotein Distribution of Lipid-Associated Nystatin and Cyclosporine Pharmaceutical Research. ,vol. 16, pp. 165- 169 ,(1999) , 10.1023/A:1011951602894
Karen E Mosier, Jiuxue Song, Gordon McKay, John W Hubbard, Jim Fang, Determination of clozapine, and its metabolites, N-desmethylclozapine and clozapine N-oxide in dog plasma using high-performance liquid chromatography Journal of Chromatography B. ,vol. 783, pp. 377- 382 ,(2003) , 10.1016/S1570-0232(02)00655-4
Matthew J. Byerly, C. Lindsay DeVane, Pharmacokinetics of Clozapine and Risperidone Journal of Clinical Psychopharmacology. ,vol. 16, pp. 177- 187 ,(1996) , 10.1097/00004714-199604000-00010
William G Honer, Allen E Thornton, Eric YH Chen, Raymond CK Chan, Jessica OY Wong, Andrea Bergmann, Peter Falkai, Edith Pomarol-Clotet, Peter J McKenna, Emmanuel Stip, Richard Williams, G William MacEwan, Kishor Wasan, Ric Procyshyn, None, Clozapine alone versus clozapine and risperidone with refractory schizophrenia. The New England Journal of Medicine. ,vol. 354, pp. 472- 482 ,(2006) , 10.1056/NEJMOA053222
Jiri Horacek, Vera Bubenikova-Valesova, Milan Kopecek, Tomas Palenicek, Colleen Dockery, Pavel Mohr, Cyril Höschl, None, Mechanism of Action of Atypical Antipsychotic Drugs and the Neurobiology of Schizophrenia CNS Drugs. ,vol. 20, pp. 389- 409 ,(2006) , 10.2165/00023210-200620050-00004
Fang Ma, Chyan E Lau, Determination of clozapine and its metabolite, N-desmethylclozapine, in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 712, pp. 193- 198 ,(1998) , 10.1016/S0378-4347(98)00148-0
A.M Almeida, M.M Castel-Branco, A.C Falcão, Linear regression for calibration lines revisited: weighting schemes for bioanalytical methods. Journal of Chromatography B. ,vol. 774, pp. 215- 222 ,(2002) , 10.1016/S1570-0232(02)00244-1
J. Ananth, S. Parameswaran, B. Hara, Drug Therapy in Schizophrenia Current Pharmaceutical Design. ,vol. 10, pp. 2205- 2217 ,(2004) , 10.2174/1381612043384042
AHSAN Y. KHAN, SHELDON H. PRESKORN, Examining concentration-dependent toxicity of clozapine: role of therapeutic drug monitoring. Journal of Psychiatric Practice. ,vol. 11, pp. 289- 301 ,(2005) , 10.1097/00131746-200509000-00003
Ric M. Procyshyn, Nathan B. Kennedy, Samantha Marriage, Kishor M. Wasan, Plasma Protein and Lipoprotein Distribution of Clozapine American Journal of Psychiatry. ,vol. 158, pp. 949- 951 ,(2001) , 10.1176/APPI.AJP.158.6.949