Detection of invasive Candida albicans infection using a specific 99mTc-labeled monoclonal antibody for the C. albicans germ tube

摘要: Accurate diagnosis is critical for effective treatment of the invasive infection by Candida albicans. Here, we investigated whether a 99m technetium (Tc)-labeled Fab’ fragment monoclonal antibody specific C. albicans germ tube could specifically identify an infection. The was used as immunogen to obtain antibodies and MAb03.2 C1–C2 with highest affinity specificity labeled 99mTc. In vitro binding assays showed that preferentially bound tubes (4.23 ± 0.17 × 102 Bq per 1 × 107 cells). These values were significantly higher than those blastospores albicans, heat-killed Aspergillus fumigatus, Staphylococcus aureus, Escherichia coli (P < 0.05). By using in vivo biodistribution planar imaging single photon emission computed tomography, demonstrated significant accumulation radioactivity albicans-infected tissues. summary, 99mTc-MAb03.2 C1–C2 able accumulate tissues, but not tissue infected A. fumigatus or bacteria sterile inflammation. This study provides new radiopharmaceutical infections.