A Quantitative Study of the Ramon Diphtheria Flocculation Reaction

摘要: Summary 1.An absolute quantitative method for the titration of diphtheria toxin and antitoxin, similar to that used by Heidelberger his co-workers precipitin reaction, has been described. 2.The results demonstrate once more antitoxin unite in than one proportion. An explanation Danysz phenomenon is given these terms. 3.Estimated values amount nitrogen per Lf unit, obtained from six titrations, varied between 0.00042 0.00048 mgm. Lf, regardless purity preparations used. 4.The maximum antitoxic antibody precipitable 300 units either as whole sera or ammonium sulfate concentrates, approximately 0.47 0.0016 unit. One highly opalescent concentrated gave a somewhat higher figure sample purified “globulin-modified” Lederle Laboratories lower figure. 5.The equivalence neutral zone lies 0.5 1.4 unit antitoxin. The ratio about 7.0 at end zone, 3.5 flocculation point 2.5 region excess. 6.The reaction closely analogous but differs it occurs only immediate neighborhood zone. 7.The calculated experiments are not expected undergo significant change even though purer toxins antitoxins may be used, provided purification process does cause splitting degradation molecules.