Induction Effects of Bacteroides fragilis Derived Outer Membrane Vesicles on Toll Like Receptor 2, Toll Like Receptor 4 Genes Expression and Cytokines Concentration in Human Intestinal Epithelial Cells.

摘要: Objective Gastrointestinal (GI) tract, like other mucosal surface, is colonized with a microbial population known as gut microbiota. Outer membrane vesicles (OMVs) which are produced by gram negative bacteria could be sensed Toll receptors (TLRs). The interaction between microbiota and TLRs affects homeostasis immune responses. In this study, we evaluated TLR2, TLR4 genes expression cytokines concentration in Caco-2 cell line treated Bacteroides fragilis (B. fragilis) its OMVs. Materials methods experimental OMVs were extracted using sequential centrifugation their physicochemical properties part of quality control assessment. cells B. (180 350 μg/ml). Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed to assess TLR2 mRNA levels. Pro-inflammatory (IFNᵧ) anti-inflammatory (IL- 4 IL-10) ELISA. Results significantly decreased slightly increased levels line. level at 180 μg/ml Conversely, the OMVs, while it Furthermore, IFNᵧ concentration, respectively. Anti-inflammatory Conclusion have pivotal role cross talk host especially modulation system. Based on last studies immunomodulatory effect derived our results, postulate that possible candidates for reduction