Human colonic intraepithelial and lamina proprial lymphocytes: cytotoxicity in vitro and the potential effects of the isolation method on their functional properties.

摘要: Colonic mucosal lymphoid cells, selectively enriched for intraepithelial (IEL) or lamina proprial lymphocytes (LPL), were isolated by sequential EDTA-collagenase treatment of resected human colons. Cytotoxic activities colonic and peripheral blood cells (PBL) tested in three different assays, using chicken erythrocytes (CRBC) Chang as targets. Antibody-dependent cell-mediated cytotoxicity (ADCC) PHA-induced (MICC) both targets shown all the isolates PBL, was spontaneous (SCMC) cells. However, no SCMC ADCC found with LPL, IEL showed minimal activity either assay. LPL demonstrated MICC but, contrasting PBL MCC CRBC. No significant differences between cytotoxic capabilities from patients inflammatory bowel disease those other diseases. Importantly, control studies that CRBC reduced collagenase used isolation, LPL. Also, EDTA. In contrast, neither EDTA nor Both forms induced variable degrees cell losses PBL. By analogy, it can be implied isolation intestinal mononuclear may influence some their vitro. This raises an important caveat interpretation such studies.