Electrochemical detection of tobramycin or gentamicin according to the European Pharmacopoeia analytical method

摘要: Tobramycin and gentamicin are two aminoglycosidic antibiotics used in lung infection, ophthalmic treatments as well skin infections. Pharmaceutical companies which produce remedies containing tobramycin need an analytical method for their internal quality control. For several years a simple chromatographic based on anion exchange separation coupled with amperometric detection was proposed aminoglycosides. This approach partially the last edition of European Pharmacopoeia (EP) analysis. In fact they use integrated pulsed (IPAD) gold electrode while is obtained polymeric wide pore reversed phase instead alkaline conditions. Such coupling seems to be cumbersome not so easy realize reproduce from one laboratory another. Besides, described lacks some details important waveform steps duration. Unfortunately control (QC) laboratories have exactly EP, complained about troubles. Therefore, EP authors published recently paper regarding guidelines good practice application, but suggestion yet resolutive. our work we evaluated eluent composition kind cell, diameter cell volume. Mainly optimized waveform. addition, analysis another explored order achieve better efficiency separate all impurities confirming effectiveness detection. The conditions seem allow analyst operate conformity method.