Production and purification of a novel extracellular lipase from Alternaria brassicicola
作者： Philippe Berto , Lionel Belingheri , Bertrand Dehorter
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摘要: Alternaria brassicicola produced higher quantities (3.2 U/ml) of an inducible extracellular lipase (EC 3.1.1.3) in shaken synthetic medium supplemented with 20 mM methyloleate. After purification, the M r was determined as 80 kDa by SDS-PAGE and estimated at 85 using gel filtration, which suggest that enzyme may be a monomer. The optimum pH temperature for activity were 9.0 25oC, respectively. Using umbelliferone esters, shown highly specific towards substrate long-chain unsaturated fatty acid.
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