Animal Models of Metastasis

摘要: Tumour metastasis is a complex and dynamic process that estimated to be associated with over 90% of cancer-related deaths. The various stages the metastatic cascade are made up interactions between tumour cells, solid microenvironment, host normal tissue. While our understanding cell migration invasion has been greatly improved using variety in vitro culture systems, inherent complexity entire best studied animal models. There several vivo models amenable studying metastasis, this Chapter will focus on some more notable commonly used field. We first consider zebrafish as burgeoning model for particularly well-suited intravital imaging labeled cells study mechanisms underlying dissemination. then discuss selected genetically engineered mouse cancer, which powerful tools elucidate oncogenic potential genes interest. Murine xenografts also considered traditional “gold standard” research. have included descriptions strategies artificially seed murine tissues proliferation survival these tissues, we provide an overview quantitative methods development spontaneous metastases from xenografts. conclude use patient-derived (PDXs), becoming increasingly common validate experimental findings established other systems effort enhance clinical applicability data.