Touchdown digital polymerase chain reaction for quantification of highly conserved sequences in the HIV-1 genome.
作者： Ward De Spiegelaere , Eva Malatinkova , Maja Kiselinova , Pawel Bonczkowski , Chris Verhofstede
关键词:
摘要: Digital polymerase chain reaction (PCR) is an emerging absolute quantification method based on the limiting dilution principle and end-point PCR. This methodology provides high flexibility in assay design without influencing quantitative accuracy. article describes to quantify HIV DNA that targets a highly conserved region of HIV-1 genome hampers optimal probe design. To maintain specificity allow binding hydrolysis with low melting temperature, two-stage touchdown PCR was designed first round amplification at temperature subsequent accumulation fluorescence.
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core.ac.uk 参考文章(18)
Ward De Spiegelaere, Mario Van Poucke, Wim Van den Broeck, Pieter Cornillie, Christian Burvenich, Luc Peelman, Quantitative mRNA expression analysis in kidney glomeruli using microdissection techniques. Histology and Histopathology. ,vol. 26, pp. 267- 275 ,(2011) , 10.14670/HH-26.267
Vagner Bernardo, Luis Felipe Ribeiro Pinto, Rodolpho Mattos Albano, Gene expression analysis by real-time PCR: experimental demonstration of PCR detection limits. Analytical Biochemistry. ,vol. 432, pp. 131- 133 ,(2013) , 10.1016/J.AB.2012.09.029
Peter Messiaen, Ward De Spiegelaere, Jose Alcami, Karen Vervisch, Petra Van Acker, Bruno Verhasselt, Pieter Meuwissen, Esther Calonge, Nuria Gonzalez, Felix Gutierrez-Rodero, Carmen Rodriguez-Martín, Erica Sermijn, Bruce Poppe, Dirk Vogelaers, Chris Verhofstede, Linos Vandekerckhove, Characterization of LEDGF/p75 Genetic Variants and Association with HIV-1 Disease Progression PLoS ONE. ,vol. 7, pp. e50204- ,(2012) , 10.1371/JOURNAL.PONE.0050204
Konstantinos Kesanopoulos, Georgina Tzanakaki, Stamatina Levidiotou, Caroline Blackwell, Jenny Kremastinou, Evaluation of touch-down real-time PCR based on SYBR Green I fluorescent dye for the detection of Neisseria meningitidis in clinical samples Fems Immunology and Medical Microbiology. ,vol. 43, pp. 419- 424 ,(2005) , 10.1016/J.FEMSIM.2004.10.011
Renée M. van der Sluis, Thijs van Montfort, Mireille Centlivre, Nick C.T. Schopman, Marion Cornelissen, Rogier W. Sanders, Ben Berkhout, Rienk E. Jeeninga, William A. Paxton, Georgios Pollakis, Quantitation of HIV-1 DNA with a sensitive TaqMan assay that has broad subtype specificity. Journal of Virological Methods. ,vol. 187, pp. 94- 102 ,(2013) , 10.1016/J.JVIROMET.2012.09.019
Deniz Pekin, Yousr Skhiri, Jean-Christophe Baret, Delphine Le Corre, Linas Mazutis, Chaouki Ben Salem, Florian Millot, Abdeslam El Harrak, J Brian Hutchison, Jonathan W Larson, Darren R Link, Pierre Laurent-Puig, Andrew D Griffiths, Valérie Taly, None, Quantitative and sensitive detection of rare mutations using droplet-based microfluidics Lab on a Chip. ,vol. 11, pp. 2156- 2166 ,(2011) , 10.1039/C1LC20128J
Ward De Spiegelaere, Tim Erkens, Jurgen De Craene, Christian Burvenich, Luc Peelman, Wim Van den Broeck, Elimination of amplification artifacts in real-time reverse transcription PCR using laser capture microdissected samples. Analytical Biochemistry. ,vol. 382, pp. 72- 74 ,(2008) , 10.1016/J.AB.2008.07.004
Benjamin J. Hindson, Kevin D. Ness, Donald A. Masquelier, Phillip Belgrader, Nicholas J. Heredia, Anthony J. Makarewicz, Isaac J. Bright, Michael Y. Lucero, Amy L. Hiddessen, Tina C. Legler, Tyler K. Kitano, Michael R. Hodel, Jonathan F. Petersen, Paul W. Wyatt, Erin R. Steenblock, Pallavi H. Shah, Luc J. Bousse, Camille B. Troup, Jeffrey C. Mellen, Dean K. Wittmann, Nicholas G. Erndt, Thomas H. Cauley, Ryan T. Koehler, Austin P. So, Simant Dube, Klint A. Rose, Luz Montesclaros, Shenglong Wang, David P. Stumbo, Shawn P. Hodges, Steven Romine, Fred P. Milanovich, Helen E. White, John F. Regan, George A. Karlin-Neumann, Christopher M. Hindson, Serge Saxonov, Bill W. Colston, High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number Analytical Chemistry. ,vol. 83, pp. 8604- 8610 ,(2011) , 10.1021/AC202028G
Matthew C. Strain, Douglas D. Richman, New assays for monitoring residual HIV burden in effectively treated individuals Current Opinion in Hiv and Aids. ,vol. 8, pp. 106- 110 ,(2013) , 10.1097/COH.0B013E32835D811B
Chih-Hui Lin, Yu-Chieh Chen, Tzu-Ming Pan, Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay PLoS ONE. ,vol. 6, pp. e29101- ,(2011) , 10.1371/JOURNAL.PONE.0029101